Anti-EN2 Antibody

Anti-EN2 Antibody LS-B3477

EN2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3477 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3477 was determined to be 5 ug/ml. Learn more

EN2 Antibody Details

 

 

Target:

Human EN2

 

Host:

Rabbit

 

Reactivity:

Dog, Human, Mouse, Rat

 

Clonality:

IgG Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry (IHC)

 

Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml)

 

Western blot (WB) (0.05 µg/ml)

 

Optimal dilution to be determined by the researcher.

 

EN2 Antibody Specificity

 

Immunogen:

EN2 antibody was raised against synthetic peptide from C-terminus of human EN2 (NP_001418.2, P19622). Percent identity by BLAST analysis: Mouse, Rat, Horse, Guinea pig (100%); Xenopus (84%).

 

Epitope:

C-Terminus

 

Specificity:

Human EN2

EN2 Antibody Packaging

 

Presentation:

Lyophilized from PBS with 2% sucrose

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.