Anti-EIF4EBP2 / 4EBP2 Antibody

Anti-EIF4EBP2 / 4EBP2 Antibody LS-B3749

EIF4EBP2 / 4EBP2 Rabbit anti-Human Polyclonal (aa99-120) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3749 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3749 was determined to be 10 ug/ml. Learn more

EIF4EBP2 / 4EBP2 Antibody Details

 

 

Target:

Human EIF4EBP2 / 4EBP2

 

Host:

Rabbit

 

Reactivity:

Bovine, Canine, Human, Mouse, Porcine, Rat

 

Clonality:

 Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Western blot (WB) (4)

 

Optimal dilution to be determined by the researcher.

 

EIF4EBP2 / 4EBP2 Antibody Specificity

 

Immunogen:

EIF4EBP2 / 4EBP2 antibody was raised against human PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH. This sequence is 96% homologous to mouse PHAS-11 over these residues.

 

Epitope:

aa99-120

 

Specificity:

Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.

EIF4EBP2 / 4EBP2 Antibody Packaging

 

Presentation:

BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

 

Restrictions:

For research use only.