Anti-DNA-PKcs Antibody

Anti-DNA-PKcs Antibody LS-B3750

DNA-PKcs Rabbit anti-Human Polyclonal (aa4085-4096) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3750 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3750 was determined to be 10 ug/ml. Learn more

DNA-PKcs Antibody Details

 

 

Target:

Human DNA-PKcs

 

Host:

Rabbit

 

Reactivity:

Hamster, Human, Mouse, Rat

 

Clonality:

 Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Western blot (WB) (2)

 

Optimal dilution to be determined by the researcher.

 

DNA-PKcs Antibody Specificity

 

Immunogen:

DNA-PKcs antibody was raised against an 11 residue synthetic peptide (C)GRTWEGWEPWM based on the human DNA-PKcs (residues 4085-4096) with the cysteine (C) residue added and the peptide coupled to KLH.

 

Epitope:

aa4085-4096

 

Specificity:

Detects a >350 kD protein, corresponding to the apparent molecular mass of DNA-PKcs on SDS-PAGE immunoblots. Also detects an ~180 kD band, believed to be a processed form of the catalytic subunit.

DNA-PKcs Antibody Packaging

 

Presentation:

PBS, pH 7.2, 50% glycerol, 0.09% azide

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

 

Restrictions:

For research use only.