Anti-D5 Dopamine Receptor (extracellular)
- 貨號 Cat. No.
- ADR-005
- 規格 Size
- 50 μl 0.2ml
Description
Anti-D5 Dopamine Receptor (extracellular)
| Product#: | ADR-005 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
PeptideEEGWELEGRTENC corresponding to amino acid residues 199-211 of rat D5 dopamine receptor (Accession P25115). |
 |
| Putative epitope location: |
Extracellular, 2nd loop. |
| Homology: |
| Mouse-12/13 residues identical. Unlikely to recognize human samples. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat striatum membranes: 1. Anti-D5 Dopamine Receptor (extracellular)antibody (#ADR-005), (1:200). 2.Anti-D5 Dopamine Receptor (extracellular), preincubated with the control peptide antigen. |
 | Western blot analysis of mouse brain membranes: 1. Anti-D5 Dopamine Receptor (extracellular)antibody (#ADR-005), (1:200). 2.Anti-D5 Dopamine Receptor (extracellular), preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
Immunohistochemical staining of D5 receptor in rat striatum. A. Perfusion-fixed brain frozen sections were stained with Anti-D5 Dopamine Receptor (extracellular) antibody (#ADR-005) (1:100)(green fluorescence). D5 receptor appears in a subset of striatal neurons and in the striatal matrix. B. Staining of the same section with calbindin D28k (red fluorescence), a marker of interneurons. C. Merging the two images demonstrates that D5 receptor partially overlaps with the population of calbindin containing striatal interneurons. Blue is DAPI counterstain. |
The D5 Dopamine Receptor (D5 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinsonî–¸ disease and schizophrenia.1
All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1
The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1
The D5 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex, hippocampus and striatum. In the periphery the D5 receptor has been localized in the adrenal cortex, kidney and intestinal tract.
The exact physiological function of the D5 receptor subtype remains poorly understood. Studies with D5 receptor knock out mice have shown no overt alterations in locomotor or cognitive functions. However, knock out mice do develop severe hypertension suggesting a role of D5 receptor in the modulation of neuronal pathways regulating blood pressure responses.2, 3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 2nd extracellular loop of the rat D5 receptor. Anti-D5 Dopamine Receptor (extracellular) antibody (#ADR-005) can be used in Western blot and immunohistochemical applications, and recognizes D5 receptor from rat and mouse samples