Anti-D3 Dopamine Receptor (extracellular)
- 貨號 Cat. No.
- ADR-003
- 規格 Size
- 50 μl 0.2ml
Description
Anti-D3 Dopamine Receptor (extracellular)
| Product#: | ADR-003 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit polyclonal. |
| Epitope: |
PeptideCGAENSTGVNRARPH corresponding to amino acid residues 15-29 of rat D3 Dopamine receptor (Accession P19020). |
 |
| Putative epitope location: |
Extracellular, N-terminus. |
| Homology: |
| Mouse- identical. Human- 12/15 residues identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat whole brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes: 1, 2. Anti-D3 Dopamine Receptor (extracellular)antibody (#ADR-003), (1:200). 3, 4.Anti-D3 Dopamine Receptor (extracellular), preincubated with the control peptide antigen. |
 | Western blot analysis of mouse brain membranes: 1. Anti-D3 Dopamine Receptor (extracellular)antibody (#ADR-003), (1:200). 2.Anti-D3 Dopamine Receptor (extracellular), preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
Expression of D3 Dopamine receptor in rat striatum. Perfusion-fixed frozen rat brain sections were stained with Anti-D3 Dopamine Receptor (extracellular) antibody (#ADR-003) (1:100). A. D3 Dopamine receptor appears in a subset of striatal neurons (green fluorescence). B. The same section was stained for parvalbumin (red fluorescence), a marker of interneurons. C. Merging the two images demonstrates that D3 Dopamine receptor localization includes parvalbuminergic striatal interneurons. Nuclei were stained with DAPI (blue) as countestain. |
The D3 Dopamine Receptor (D3 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinsonî–¸ disease and schizophrenia.1
All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1
The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1
The D3 receptor distribution in the brain is relatively restricted to limbic areas such as striatum, islands of Calleja and olfactory tubercle. In the periphery, it is expressed in the kidney particularly in proximal tubules.1
The exact physiological function of the D3 receptor remains to be fully elucidated. In studies using D3 receptor knockout mice, the most prominent dysfunction was the development of rennin-dependent hypertension. Potential roles in reinforcement and reward behaviors have also been suggested as well as roles in neuropsychiatric disorders such as drug abuse and schizophrenia.2
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus domain of the rat D3 receptor. Anti-D3 Dopamine Receptor (extracellular) antibody (#ADR-003) can be used in Western blot and immunohistochemical applications, and recognizes D3 receptor from rat and mouse samples.