Anti-d-Opioid Receptor (extracellular)
- 貨號 Cat. No.
- AOR-014
- 規格 Size
- 50 μl 0.2ml
Description
Anti-d-Opioid Receptor (extracellular)
| Product#: | AOR-014 |
| Sizes: |
50µl |
0.2ml |
| Host: |
Rabbit. |
| Epitope: |
Peptide (C)ELVP SARAE LQSSP LVN, corresponding to amino acid residues 2-18 of the mouse d-opioid receptor (Accession P32300). |
 |
| Putative epitope location: |
N-terminus, extracellular. |
| Homology: |
Rat ?13/17 amino acid residues identical. Human ?will not recognize the human epitope. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of a rat cortex lysate: 1. Anti-d-Opioid Receptor (extracellular) antibody (#AOR-014), (1:200). 2. Anti-d-Opioid Receptor (extracellular) antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of d opioid receptor (DOR-1) in rat spinal cord |
 |
| Immunohistochemical staining of rat spinal cord frozen section using Anti-d-Opioid Receptor (extracellular) antibody (#AOR-014), (1:100) followed by goat-anti-rabbit Alexa Fluor 488 (green). Staining is present in neuronal cell bodies (white arrows). Hoechst 33342 (blue) is used as counterstain. |
Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (?-opioid (MOP) receptor, delta (d)-opioid (DOP) receptor, and kappa (k)-opioid (KOP) receptor.1 Recently, the nociceptin/orphanin FQ(N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic ? d, and k receptors.2
The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins.1
All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the ?opioid receptor, whereas ?, d-, and k-receptors participate in the control of pain at the spinal level.3 The opioid receptors also mediate the mood-altering properties of opioids.4 Cross-talk between m- and d- opioid receptors was demonstrated when subeffective doses of d-opioid receptors agonists modulated m-mediated analgesia.5
The d receptors are discretely distributed in the central nervous system (CNS), with a prominent gradient of receptor density from high levels in forebrain structures to relatively low levels in most hindbrain regions.2
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the mouse d-opioid receptor. Anti-d-Opioid Receptor (extracellular) antibody (#AOR-014) can be used in Western blot analysis and should recognize d-opioid receptor from mouse and rat samples.