Anti-CTSB / Cathepsin B Antibody

Anti-CTSB / Cathepsin B Antibody LS-B3313

CTSB / Cathepsin B Rabbit anti-Human Polyclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3313 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3313 was determined to be 1:100. Learn more

CTSB / Cathepsin B Antibody Details

 

 

Target:

Human CTSB / Cathepsin B

 

Host:

Rabbit

 

Reactivity:

Human, Mouse, Rat

 

Clonality:

 Polyclonal

 

Purification:

Affinity Chromatography

 

Applications:

 

Immunohistochemistry (IHC) (10 - 20 µg/ml)

 

Immunohistochemistry - Paraffin (IHC-P) (1:100)

 

Western blot (WB) (0.5 - 4 µg/ml)

 

Optimal dilution to be determined by the researcher.

 

CTSB / Cathepsin B Antibody Specificity

 

Immunogen:

CTSB antibody was raised against synthetic peptide surrounding amino acid 322 of human cathepsins B.

 

Specificity:

The purified antibody detects both the proform (37-43 kD) and the mature form (25 kD) of cathepsin B. It reacts with human and mouse and in a lesser extent with rat samples.

CTSB / Cathepsin B Antibody Packaging

 

Presentation:

PBS, pH 7.2, 30% glycerol, 0.5% BSA, 0.1% Thimerosal.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.