Anti-CRABP1 Antibody

Anti-CRABP1 Antibody LS-B3590

CRABP1 Mouse anti-Human Monoclonal (AT1A1) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3590 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3590 was determined to be 5 ug/ml. Western Blot: Extracts of mouse eye (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Learn more

CRABP1 Antibody Details

Target: Human CRABP1

Host: Mouse

Reactivity: Human, Mouse

Clonality: IgG2b,k Monoclonal [AT1A1] 

Purification: Protein G Column

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1:500 - 1:1000)

ELISA

Optimal dilution to be determined by the researcher.

 

CRABP1 Antibody Specificity

Immunogen: CRABP1 antibody was raised against recombinant human CRABP1 (1-137aa) purified from E. coli

Specificity: human CRABP1 (1-137aa)

CRABP1 Antibody Packaging

Presentation: PBS, pH 7.4, 0.1% sodium azide

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For research use only.