Anti-Complement C4c Antibody

Anti-Complement C4c Antibody LS-B4228

Complement C4c Sheep anti-Human Polyclonal Antibody

An IHC-plusTM Antibody

 Applications: Suitable for use in Immunohistochemistry, and gel techniques, (RID, double diffusion, IEP and rocket IEP). Other applications not tested. Recommended Dilution: RID, double diffusion and IEP: Use 3% PEG 6000 with 1.2% agarose in TBE or Tris-barbital pH >8.2 buffer. RID & Rocket RID: 1ul/cm2 gel vs. 5ul neat- 1:5 dilution human plasma. Double Diffusion: 10ul antiserum/5ul plasma IEP: 100ul antiserum /5ul plasma Optimal dilutions to be determined by the researcher. Learn more

Complement C4c Antibody Details

 

 

Target:

Human Complement C4c

 

Host:

Sheep

 

Reactivity:

Cat, Dog, Guinea pig, Horse, Human, Mouse, Rat

 

Non-Reactivity:

Bovine, Chicken, Goat, Rabbit

 

Clonality:

IgG Polyclonal

 

Purification:

DEAE-column Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (5)

 

Immunodiffusion (ID)

 

Immunoelectrophoresis (IE)

 

Optimal dilution to be determined by the researcher.

 

Complement C4c Antibody Specificity

 

Immunogen:

Complement C4c antibody was raised against human C4 purified from plasma, shown to be pure by SDS-PAGE.

 

Specificity:

Recognizes human Complement C4. Gives a single arc when tested by IEP and 2D-IEP against fresh human plasma. Identity has been confirmed by double diffusion (Ouchterlony) vs human plasma and a known anti-human C4.

Complement C4c Antibody Packaging

 

Presentation:

GBS, pH 7.4, 0.1% EACA, 1 mM EDTA, 0.01% Benzamidine, 0.1% sodium azide

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

 

Restrictions:

For research use only.