Anti-Collagen IV Antibody

Anti-Collagen IV Antibody LS-B3654

Collagen IV Rabbit anti-Human Polyclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3645 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40; fresh frozen or acetone fixed specimens. Dot Blotting: 1:300; not recommended for Western Blots. Learn more

Collagen IV Antibody Details

 

 

Target:

Human Collagen IV

 

Host:

Rabbit

 

Reactivity:

Human

 

Clonality:

IgG Polyclonal

 

Purification:

Affinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (1:100)

 

Immunohistochemistry - Frozen (IHC-Fr)

 

Immunofluorescence (IF)

 

ELISA (1:3000)

 

Optimal dilution to be determined by the researcher.

Failed Applications:

Western blot (WB)

 

Collagen IV Antibody Specificity

 

Immunogen:

Collagen IV antibody was raised against human placental collagen type IV.

 

Specificity:

Human Type IV collagen. Cross-reactivity: Human collagen type I: 1% Human collagen type II: <0.5%, Human collagen type III: <0.5%, Human collagen type IV: 100%, Human collagen type V: 7%, Human fibronectin: <0.5%, Human albumin: <0.5%, Human immunoglobulin <0.5%.

Collagen IV Antibody Packaging

 

Presentation:

PBS, mannitol, dextran, no preservatives.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.