Anti-Collagen I Antibody

Anti-Collagen I Antibody LS-B3653

Collagen I Rabbit anti-Human Polyclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3653 was determined to be 1:100. ELISA on human collagen type I: 1:4000. Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue: 1:10-1:40. Learn more

Collagen I Antibody Details

 

 

Target:

Human Collagen I

 

Host:

Rabbit

 

Reactivity:

Human

 

Clonality:

IgG Polyclonal

 

Purification:

Ammonium Sulfate Precipitation

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (1:100)

 

Immunofluorescence (IF)

 

ELISA

 

Optimal dilution to be determined by the researcher.

Failed Applications:

Western blot (WB)

 

Collagen I Antibody Specificity

 

Immunogen:

Collagen I antibody was raised against human placental collagen Type I.

 

Specificity:

Human collagen type. Cross-reactivity: Human collagen type I: 100%, Human collagen type II: <0.5%, Human collagen type III: < 25%, Human collagen type IV: <0.5%, Human collagen type V: <0.5%, Human fibronectin: <0.5%, Human albumin: <0.5%, Human immunoglobulin: <0.5%.

Collagen I Antibody Packaging

 

Presentation:

0.15 M sodium chloride, 10 mM sodium phosphate, pH 7.5, 0.1% Mannitol, 0.1% Dextran as stabilizers. No preservative.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.