Anti-CNGA2
- 貨號 Cat. No.
- APC-045
- 規格 Size
- 50 μl 0.2ml
Description
Anti-CNGA2
| Product#: | APC-045 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)KQNH EDDYL SDGIN TPEP, corresponding to amino acid residues 643-660 of rat CNGA2. (Accession Q00195). |
 |
| Putative epitope location: |
| Intracellular, C-terminus. |
| Homology: |
Mouse - identical; rabbit - 15/18 amino acid residues identical; human, bovine - 14/18 amino acid residues identical. |
Applications:
| Immunohistochemistry: |
Expression of CNGA2 in rat cerebellum |
 |
Immunohistochemical staining of CNGA2 channel in rat cerebellum using Anti-CNGA2 antibody (#APC-045). A, CNGA2 channel (red) appears in Purkinje cells (vertical arrows) and in astrocytic fibers (horizontal arrows) traversing the cerebellar molecular layer (Mol). B, staining of astrocytes with mouse anti glial fibrillary acidic protein (GFAP, green) demonstrates the full distribution of astrocytic fibers (horizontal arrows) in the cerebellum. C, Confocal merge of CNGA2 and GFAP. Expression of CNGA2 in Mouse Cerebrum |
 |
Immunohistochemical staining of CNGA2 channel in mouse cerebrum using Anti-CNGA2 antibody (#APC-045). A, CNGA2 channel (red) appears in cells lining up the wall of the lateral ventricle (LV) (horizontal arrows). B, staining of astrocytes with mouse anti glial fibrillary acidic protein (GFAP, green) demonstrates penetration of astrocytic fibers (vertical arrows) into the wall of the lateral ventricle. C, Confocal merge of CNGA2 and GFAP. |
| Immunocytochemistry: |
Expression of CNGA2 in Rat Cerebellum |
 |
Immunocytochemical staining of rat cerebellum primary culture. Paraformaldehyde-fixed and permeabilized cerebellum cells were stained as described. A-F. Immunocytochemical staining using Anti-CNGA2 antibody (#APC-045), (1:100) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. Magnification: A-C: x20 D-F: x100 |
| References: |
| 1. Biel, M. et al.(1999) Rev. Physiol. Biochem. Pharmacol. 135, 151. |
| 2. Kramer, R. H. and Molokanova, E. (2001) J. Exp. Biol. 204, 2921. |
| 3. Matulef, K. (2003) Annu. Rev. Cell. Dev. Biol. 19, 23. |
| 4. www.iuphar-db.org. |
| 5. Molday, R.S. (1996) Curr. Opin. Neurobiol. 6, 445. |
| 6. Kingston, P.A. et al.(1996) Proc. Natl. Acad. Sci. USA. 93, 10440. |
| 7. Podda, M.V. et al. (2005) Neuroreport,16, 1939. |
Cyclic nucleotides are important second messengers in many cellular functions such as visual transduction, and relaxation of smooth muscle cells. Cyclic nucleotides exert their cellular functions through three major classes of cellular receptors, one of them is the cyclic nucleotide-gated (CNG) channels.1 The CNG channels are non-selective cation channels facilitating the influx of Na+ and Ca2+ ions, following activation by intracellular cyclic nucleotides.2 In vertebrates, six members of the CNG channel family were identified and grouped according to sequence homology into two subtypes, CNGA and CNGB. To date, four types of the a subunits (CNGA1-4) and two b subunits (CNGB1, CNGB3) have been characterized.3-4
Native CNG channels are composed of a and b subunits in a tetrameric configuration.
Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains, but are also modulated by other factors including phosphorylation and calmodulin.5 In a heterologous expression system, only the a subunits are capable of forming functional homomeric channels.
CNG ion channels are essential in visual and olfactory signal transduction.
Native CNG channels are composed of a and b subunits in a tetrameric configuration.
Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains, but are also modulated by other factors including phosphorylation and calmodulin.5 In a heterologous expression system, only the a subunits are capable of forming functional homomeric channels.
CNG ion channels are essential in visual and olfactory signal transduction.
CNG channels were originally detected in rod and cone photoreceptors and olfactory receptor cells, where they mediate the transduction of external sensory stimuli into neuronal activity.6
CNGA2 is predominantly expressed in olfactory neurons (the olfactory type receptor). However, electrophysiological and molecular data indicate that CNGA1, and especially CNGA2, are widely distributed and functionally active in many regions of the brain, including the hippocampus, cerebral cortex, cerebellum, and brainstem.7
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the CNGA2 channel. Anti-CNGA2 (#APC-045) can be used for western blotting, immunohistochemical and immunocytochemistry applications. It has been designed to recognize CNGA2 from human, rat and mouse samples.