Anti-CLC-5

 

Product#:

ACL-003

Sizes:

50 ml

0.2 ml

CLC5 is a member of the voltage-dependent Cl^- channel family that includes nine known members.  

Based in sequence similarity, CLC5 is closely linked to the CLC3 and CLC4 subunits.  

Mutations in the CLC5 gene cause Dent's disease a X-linked renal disorder.  

CLC-5 is mainly found in intracellular vesicles, particularly in early endosomes vesicles,  

where it is believed to contribute to their acidification.  

The channel is mainly expressed in the kidney, although it has been found also in small  

intestine, brain and liver.

Host:

Rabbit.

Epitope:

Peptide CDYENHFNTSKGGEL, corresponding to amino acid residues 401-415 of rat CLC5   (Accession number P51796 ).

Putative epitope location:

Extracellular.

Homology:

Mouse - identical; human- 14/15 residues identical. Specificity:     The antibody is specific for CLC5; it does not crossreact with other CLC family proteins.

Reactivity Confirmed:

Rat.

Applications:

Western Blotting:

Western blotting of rat kidney lysate: 1.       Anti-CLC-5 antibody (#ACL-003)(1:200). 2.   Anti-CLC-5 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:

Rat kidney section.

Immunohistochemical staining of CLC5 in rat kidney using Anti-CLC5 antibody (#ACL-003).   CLC5 was visualized with immuno-peroxidase methods and final brown-black diaminobenzidine   color product (arrows in A). The counterstain is cresyl violet.   When the antibody was pre-incubated with the immunogen, staining was blocked (B).

References:

1. Jentsch, T.J.et al. (2002) Physiol. Rev. 82, 503.

2. Piwon, N. et al. (2000) Nature 408, 369.