Anti-CLC-3
- 貨號 Cat. No.
- ACL-001
- 規格 Size
- 50 μl 0.2ml
Description
Anti-CLC-3
| Product#: | ACL-001 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
| GST fusion protein with residues 592-661 of rat CLC-3 (Accession P51792). |
| Putative epitope location: |
| Intracellular, near the C-terminus. |
| Homology: |
Mouse - identical; human, rabbit, guinea pig - 69/70 residues identical; Xenopus laevis - 61/70 residues identical. |
| Reactivity Confirmed: |
Rat. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes. 1. Anti-CLC-3 antibody (#ACL-001)(1:200). 2. Anti-CLC-3, preincubated with a control antigen. |
 | Western blotting of membranes from Xenopus oocytes, expressing CLC-3, CLC-4, and CLC-5, using Anti-CLC-3 antibody (#ACL-001) (kindly provided by Prof. Jordi Ehrenfeld, University of Nice). |
| References: |
| 1. Jentsch, T.J. et al. (2002) Physiol. Rev. 82, 503. |
| 2. Babini, E. and Pusch, M. (2004) Physiology 19, 293. |
| 3. Stobrawa, S.M. et al. (2001) Neuron 29, 185. |
| 4. Moreland, J.G. et al. (2006) J. Biol. Chem. 281, 12277. |
CLC-3 is a member of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.
CLC channels that function in the plasma membrane are involvedin the stabilization of membrane potential and in transepithelialtransport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl-/H+ antiporters.1, 2
The functional unit of the CLC channels is a dimer with each subunit forming a proper pore.
Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2
CLC-3 is widely distributed with prominent expression in tissues of neuroectoderm origin. In the brain, it is highly expressed in the hippocampus, olfactory bulb and olfactory cortex. The channel is also prominently expressed in aortic and coronary vascular smooth muscle cells, aortic endothelial cells and tracheal and alveolar epithelial cells.
The physiological function of CLC-3 is not entirely clear, but it has been suggested that CLC-3 generates a shunt current of chloridefor v-H+-ATPases, thereby aiding the acidification of endosomesand synaptic vesicles as well as lysosomes. Disruption of the ClC-3 gene in mice causes severe neuronal loss, leading to a complete loss of the hippocampus in adult mice. In addition, CLC-3 has been shown to have a critical role in the respiratory burst and phagocytosis of polymorphonuclear cells, a key cell type of innate host defense. 3,4
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of rat CLC-3. Anti-CLC-3 antibody (#ACL-001) can be used in Western blot and immunohistochemical applications, and recognizes the CLC-3 channel from rat and mouse samples.