Anti-CLC-1

CLC-1 is a member of the voltage-dependent Cl^- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.

CLC channels that function in the plasma membrane are involvedin the stabilization of membrane potential and in transepithelialtransport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl^-/H⁺ antiporters.^{1, 2}

 

The functional unit of the CLC channels is a dimer with each subunit forming a proper pore.

Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. ^1,2

 

The ClC-1 channel is almost exclusively expressed in skeletal muscle where it has a dominant role in maintaining the membrane potential at rest and is important for repolarization of the skeletal muscle cells.

Mutations in the ClC-1 gene result in myotonia congenita, a condition in which an increase in the excitability of skeletal muscle leads to repetitive action potentials, stiffness, and delayed relaxation. The disease can be either autosomal dominant (Thomsen's disease), or recessive (Becker's myotonia).³

 

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular N-terminal domain of rat CLC-1. Anti-CLC-1 antibody (#ACL-005) can be used in Western blot applications, and recognizes the CLC-1 channel from rat samples.