Anti-CD53 Antibody

Anti-CD53 Antibody LS-B4184

CD53 Mouse anti-Human Monoclonal (MEM-53) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B4184 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4184 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Learn more

CD53 Antibody Details

 

 

Target:

Human CD53

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG1 Monoclonal [MEM-53]

 

Purification:

Protein G Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Immunohistochemistry - Frozen (IHC-Fr)

 

Western blot (WB)

 

Immunoprecipitation (IP)

 

Flow Cytometry (Flo) (1:10 - 1:25)

 

Optimal dilution to be determined by the researcher.

 

CD53 Antibody Specificity

CD53 Antibody Packaging

 

Presentation:

PBS, pH 7.4, 0.09% sodium azide.

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

 

Restrictions:

For research use only.