Anti-CD163 Antibody

Anti-CD163 Antibody LS-B3781

CD163 Mouse anti-Human Monoclonal (GHI/61) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3781 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3781 was determined to be 20 ug/ml. Tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Learn more

CD163 Antibody Details

 

 

Target:

Human CD163

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG1,k Monoclonal [GHI/61]

 

Purification:

Affinity Purified

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (20)

 

Western blot (WB)

 

Flow Cytometry (Flo)

 

Optimal dilution to be determined by the researcher.

 

CD163 Antibody Specificity

 

Immunogen:

CD163 antibody was raised against spleen cells from a case of hairy cell leukemia

 

Specificity:

CD163

CD163 Antibody Packaging

 

Presentation:

PBS, pH 7.2, <=0.09% sodium azide

 

Storage:

Store at 4°C, avoid repeated freeze thaw cycles.

 

Restrictions:

For research use only.