Anti-CD11b Antibody

Anti-CD11b Antibody LS-B3760

CD11b Rat anti-Mouse Monoclonal (M1/70.15) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3721 was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3760 was determined to be 10 ug/ml. Learn more

CD11b Antibody Details

 

 

Target:

Mouse CD11b

 

Host:

Rat

 

Reactivity:

Human, Mouse, Rabbit

 

Clonality:

IgG2b Monoclonal [M1/70.15]

 

Purification:

Ion Exchange Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Immunohistochemistry - Frozen (IHC-Fr)

 

Immunoprecipitation (IP)

 

Flow Cytometry (Flo) (1:50)

 

Optimal dilution to be determined by the researcher.

 

CD11b Antibody Specificity

 

Immunogen:

CD11b antibody was raised against t cell enriched splenocytes from B10 mice.

 

Specificity:

Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

CD11b Antibody Packaging

 

Presentation:

PBS, 0.09% sodium azide.

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.