Anti-CCND1 / Cyclin D1 Antibody

Anti-CCND1 / Cyclin D1 Antibody LS-B3452

CCND1 / Cyclin D1 Rabbit anti-Human Polyclonal (Ser90) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3452 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use in IHC with mouse samples. Learn more

CCND1 / Cyclin D1 Antibody Details

 

 

Target:

Human CCND1 / Cyclin D1

 

Host:

Rabbit

 

Reactivity:

Human, Mouse

 

Clonality:

 Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Western blot (WB) (1:50 - 1:100)

 

Immunofluorescence (IF) (1:10 - 1:50)

 

ELISA (1:1000)

 

Optimal dilution to be determined by the researcher.

 

CCND1 / Cyclin D1 Antibody Specificity

 

Immunogen:

CCND1 antibody was raised against kLH conjugated synthetic peptide corresponding to amino acid residues surrounding S90 of human Cyclin D1.

 

Epitope:

Ser90

CCND1 / Cyclin D1 Antibody Packaging

 

Presentation:

PBS, 0.09% sodium azide

 

Storage:

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

 

Restrictions:

For research use only.