Anti-CBR3 Antibody

Anti-CBR3 Antibody LS-B3589

CBR3 Mouse anti-Human Monoclonal (AT3C5) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3589 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3589 was determined to be 5 ug/ml. Western Blot: Cell lysates of HepG2 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CBR3 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Learn more

CBR3 Antibody Details

Target: Human CBR3

Host: Mouse

Reactivity: Human

Clonality: IgG1,k Monoclonal [AT3C5] 

Purification: Protein G Column

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1:1000 - 1:2000)

ELISA

Optimal dilution to be determined by the researcher.

 

CBR3 Antibody Specificity

Immunogen: CBR3 antibody was raised against recombinant CBR3 (1-277aa) purified from E. coli

Specificity: CBR3 (1-277aa)

CBR3 Antibody Packaging

Presentation: PBS, pH 7.4, 0.1% sodium azide

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For research use only.