Anti-Cav3.3

Anti-Ca_v3.3 (a1I)

Product#:

ACC-009

Sizes:

50 ml

0.2 ml

T-type Ca²⁺ channels play an important role in many cellular processes such as  

hormone secretion, neurotransmitter release and cell differentiation.  

T-type channels are also known to participate in the pacemaker activities of heart 

and neurons including thalamic neurons.¹ Three genes encoding T-type Ca²⁺ channels  

have been cloned and designated as Ca_v3.1 (a1G), Ca_v3.2 (a1H) and Ca_v3.3 (a1I).^1-3  

While Ca_v3.1 (a1G) and Ca_v3.2 (a1H) are widely expressed in various tissues, Ca_v3.3 (a1I) is primarily  

expressed in the central nervous system, where high expression was described in thalamic neurons.  

The Ca²⁺ current generated by the Ca_v3.3 channel displays much slower activation and inactivation  

compared to the currents produced by Ca_v3.1 and Ca_v3.2, suggesting it might play a different role 

in neuronal excitability.^1,4

Host:

Rabbit.

Epitope:

Peptide CNGRMPNIAKDVFTK   corresponding to amino acid residues 1053-1067 of rat Cav3.3.  (Accession number Q9Z0Y8.)

Putative epitope location:

Intracellular, between TM12-13.

Homology:

Human-14/15 residues identical.

Reactivity Confirmed:

Rat.

Applications:

Western Blotting:

Western blotting

Western blotting of rat brain membranes:  1. Anti-Cav3.3 antibody, 1:200.   2. Anti-Cav3.3 antibody, preincubated with the control peptide antigen.

References:

1. Park, J-Y. et al. (2004) J. Biol. Chem. 279, 21707.

2. Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678.

3. Montiel, A. et al. (2000) J. Biol. Chem. 275, 16530.

4. Gomora, J.C. et al. (2002) Biophysical Journal  83, 229.