Anti-Cav3.2
- 貨號 Cat. No.
- ACC-025
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Cav3.2 (a1H)
| Product#: | ACC-025 |
| Sizes: |
50 µl |
0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide CHVEGPQERARVAHS corresponding to amino acid residues 581-595 of rat Cav3.2 (Accession number Q9EQ60 ). |
 |
| Putative epitope location: |
Intracellular loop connecting D1-D2. |
| Homology: |
Mouse, bovine and canis-14/15 amino acid residues identical. Human ?13/15 amino acid residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of Cav3.2 in dorsal root ganglion (DRG) lysate: 1. Anti-Cav3.2 antibody (#ACC-025), (1:200). 2. Anti-Cav3.2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Cav3.2 in mouse cerebellum |
 |
Immunohistochemical staining of Cav3.2 in mouse cerebellum frozen section using Anti-Cav3.2 antibody (#ACC-025) (1:100). A. Cav3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with mouse anti parvalbumin (PV, green). C. Confocal merge of Cav3.2 and PV demonstrates presence of Cav3.2 adjacent to Purkinje cells. Expression of CaV 3.2 in rat dorsal root ganglia (DRG). |
 |
Immunohistochemical staining of Cav3.2 in rat DRG frozen section using Anti- CaV 3.2 antibody(#ACC-025), (1:50). Staining is specific for DRG neurons. Note that neither glial cells nor axonal fibers are stained. Hoechst 33342 is used as the counterstain. |
| Immunocytochemistry: |
Expression of Cav3.2 in rat dorsal root ganglion (DRG) primary culture |
 |
Immunocytochemical staining of Cav3.2 in rat dorsal root ganglion (DRG) primary culture. Paraformaldehyde-fixed and permeabilized DRG cells were stained as described. A, D. Immunocytochemical staining using Anti- Cav3.2 antibody (#ACC-025), (1:200) followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E. Magnification: A-C: x20 D-F: x100 |
| References: |
| 1. Park, J-Y. et al. (2004) J. Biol. Chem. 279, 21707. |
| 2. Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678. |
| 3. Monteil, A. et al. (2000) J. Biol. Chem. 275, 16530. |
| 4. Gacki鋨e F. et al. (2008) J Biol Chem. 283, 10162. |
| 5. Khosravani, H. et al. (2004) J. Biol. Chem. 279, 9681. |
| 6. Chen, C-C. et al. (2003) Science 302,1416. |
| 7. Bourinet, E. et al. (2005) EMBO J. 24, 315. |
T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation. T-type channels are also known to participate in the pacemaker activities of heart and neurons including thalamic neurons.1
Three genes encoding T-type Ca2+ channels have been cloned and designated as Cav3.2 (a1H), Cav3.1 (a1G) and Cav3.3 (a1I).1-3
The Cav3.2 (a1H) channel is widely expressed in various tissues, brain, heart, liver and testis.
Involvement of Cav3.2 Ca2+ channels in several pathologies has been described. Overexpression of the Cav3.2 channel was described in prostate cancer cells that are associated with more aggressiveness, invasiveness and poor prognosis.4 Several point mutations discovered in the Cav3.2 channel that affect gating of the channel were found to be associated with Childhood Absence Epilepsy.5 One year old mice, deficient in Cav3.2 channel, exhibited severe cardiac pathology, fibrosis, necrosis, lymphocyte infiltration and abnormal coronary function compared to wild-type mice.6
Recently, it has been demonstrated that T-type channels are expressed in DRG neurons and that small and medium-diameter primary afferent neurons in the dorsal horn expresses almost exclusively the Cav3.2 Ca2+channels. This might indicate a possible role for Cav3.2 in nociception.7
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the Cav3.2 channel. The Anti- Cav3.2 antibody (#ACC-025) can be used for Western Blot analysis, Immunohistochemical and Immunocytochemical applications. It has been designed to recognize Cav3.2 from rat, human and mouse samples.