Anti-Cav2.3
- 貨號 Cat. No.
- ACC-006
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Cav2.3
| Product#: | ACC-006 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)SASQ ERSLD EGVSI DG (CNE2), corresponding to amino acid residues 892-907 of rat CaV2.3 (Accession Q07652). |
 |
| Putative epitope location: |
Intracellular loop between II and III domains. |
| Homology: |
Mouse, rabbit, human -14/16, 12/16, and 11/16 amino acid residues identical, respectively. |
| Reactivity Confirmed: |
Rat and mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain membranes: 1. Anti CaV2.3 antibody (#ACC-006) (1:200). 2. Anti CaV2.3 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of Cav2.3 in mouse cerebellum |
 |
Immunohistochemical staining of Cav2.3 in mouse cerebellum with Anti-Cav2.3 antibody (#ACC-006). Cav2.3 (red) appeared in both soma and dendritic trees of several Purkinje cells. Staining of neurofilament 200 (green) in the same brain section demonstrates the relationship between axons passing through the granule layer and the Purkinje cells. |
| References: |
| 1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214. |
| 2. Gasparini, S. et al. (2001) J. Neurosci. 21, 8715. |
| 3. Lanzafame, A.A. et al. (2003) Receptors Channels 9, 241 |
Voltage-dependent Ca2+ channels (Cav channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1
The voltage dependent Ca2+ channels are composed of several subunits; a1, b, a2d and g.
Cav channels were originally divided into six physiological types: L, N , P, Q, R, and T type.1
The Cav2.3 (formerly named a1E) protein, forms the R type channels. R-type channels are highly sensitive to both SNX-482 (#S-500) and NiCl2. Cav3.2 channels were shown to mediate fast excitatory transmission in several synapses including the hippocampus.2
Cav2.3 channels can be modulated by the muscarinic G-protein coupled receptors.
In HEK-293 cells, expressing Cav2.3 channels, the R-type channel was shown to be both facilitated and inhibited by m1 and m2 receptors. However, inhibition was much weaker than facilitation for the m1 receptor, while for m2 receptors, inhibition was much more pronounced than activation.3
Facilitation and inhibition by the same receptors was probably exerted via different pathways as was indicated by their response to various inhibitors.3
Alomone Labs is pleased to offer an antibody raised against an intracellular epitope of the Cav2.3 Ca2+ channel.