Anti-Cav2.2
- 貨號 Cat. No.
- ACC-002
- 規格 Size
- 50 μl
Description
Anti-Cav2.2
| Product#: | ACC-002 |
| Sizes: |
| 50 ml |
| 0.2 ml |
Voltage dependent Ca2+ channels (Cav channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)RHHR HRDRD KTSAS TPA (CNB1), corresponding to amino acid residues 851-867 of rat Cav2.2 (Accession Q02294). |
 |
| Putative epitope location: |
Intracellular loop between II and III domains. |
| Homology: |
Mouse - 15/17 amino acid residues identical; human 11/17 amino acid residues identical; rabbit - 11/17 amino acid residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain membranes: 1. Anti-Cav2.2 antibody (#ACC-002) (1:200). 2. Anti-Cav2.2 antibody, preincubated with the control antigen. |
| Immunohistochemistry: |
Expression of Cav2.2 in mouse cerebellum |
 |
Immunohistochemical staining of Cav2.2 channel using Anti-Cav2.2 antibody (#ACC-002), (1:100) in mouse cerebellum. (A) Cav2.2 channel (red) appears in Purkinje cells (arrows) and is distributed diffusely in the molecular layer (Mol). (B) staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) demonstrates the restruction of Cav2.2 to cell bodies but not to dendrites in the molecular layer. |
| References: |
| 1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214. |
| 2. Beuckmann, C.T. et al. (2003) J. Neurosci. 23, 6793. |
| 3. Scheuber, A. et al. (2004) J. Neurosci. 24,10402. |
| 4. Dascal, N. (2001) Trends Endocrinol. Metab. 12, 391. |
| 5. McCleskey, E. W. et al. (1987) Proc. Natl. Acad.Sci. USA 84, 4327. |
| 6. Feng, Z. P. et al. (2001) J. Biol. Chem. 276, 15728. |
The voltage dependent Ca2+ channels are composed of several subunits; a1, b, a2d and g.
Cav channels were originally divided into six physiological types: L, N , P, Q, R, and T type.
The Cav2.2 (formally named a1B) compose the a1 poreforming subunit in the N type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, g-aminobutyric acid, acetylcholine, dopamine and noradrenaline.2
The Cav2.2 is expressed preferentially in the central nervous system, where along with Cav2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,3
The Cav2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding Gbg to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.4
w-Conotoxin GVIA (#C-300) is a specific blocker of Cav2.2 Ca2+ channels. It specifically blocks N-type Cav channels by binding to the Cav2.2 a1 subunit (a1B) and its action is only partially reversible.5,6
Alomone Labs offers Anti-Cav2.2 antibody, which is a specific, affinity purified antibody raised against an intracellular epitope of the Cav2.2 Ca2+ channel.