Anti-Cav2.1
- 貨號 Cat. No.
- ACC-001
- 規格 Size
- 50 μl
Description
Anti-Cav2.1
| Product#: | ACC-001 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)PSSP ERAPG REGPY GRE (CNA1), corresponding to residues 865-881 of rat CaV2.1 (Accession P54282). |
 |
| Putative epitope location: |
Intracellular loop between II and III domains. |
| Homology: |
Mouse-16/17 residues identical; human -10/17 residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-CaV2.1 antibody (#ACC-001) (1:200). 2. Anti-CaV2.1 preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Immunohistochemical staining of mouse cerebellum |
 |
Immunohistochemical staining of Cav2.1 channel with Anti-Cav2.1 antibody (#ACC-001) (1:100) in mouse cerebellum. (A) Cav2.1 channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (vertical arrows). (B) staining of astrocytic fibers with glial fibrillary acidic protein in the section demonstrates the location of astrocytic fibers in the molecular layer. (C) Confocal merge of Cav2.1 and GFAP. |
| References: |
| 1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214. |
| 2. Scheuber, A. et al. (2004) J. Neurosci. 24,10402. |
| 3. Ophoff, R. A. et al (1996) Cell 87, 543. |
| 4. Ishikawa, K. et al. (1997) Am. J. Hum. Genet. 61, 336. |
| 5. Tottene, A. et al. (2002) Proc. Natl. Acad. Sci. 99,13284. |
| 6. Mintz, I. M. et al. (1992) Nature 355, 827. |
| 7. Moreno, H. et al. (1997) Proc. Natl. Acad. Sci. 94, 14042. |
| 8. Bourinet, E. et al. (1999) Nat. Neurosci. 2, 407. |
Voltage dependent calcium channels (Cav channels) are pivotal players in many physiological roles such as secretion, contraction migration and excitation.1
The voltage dependent calcium channels are composed of several subunits; a1, b, a2d and g.
Cav channels were originally divided into six physiological types: L, N , P, Q, R, and T type.
The Cav2.1 (formally named a1A) compose the a1 poreforming subunit in P/Q type . It is expressed preferentially in the central nervous system where along with Cav2.2 is responsible for pre-synaptic calcium influx and neurotransmitter release.1,2
Ca2+ channel family
Mutations in the Cav2.1 have been shown to cause several neurological disorders among them are familial hemiplegic migraine, episodic ataxia type 2, and spinocerebellar ataxia type 6 (SCA6).1,3-5
Involvement of Cav2.1 in synaptic transmission was assessed by the use of w-Agatoxin IVA (#A-500), a specific blocker to the Cav2.1 channel.6 The blocking sensitivity is dependent on the a subunit isoform and on the splice variant.7,8
Alomone Labs offers a polyclonal antibody directed against an intracellular epitope of the Cav2.1 chennel.