Anti-Cav1.2
- 貨號 Cat. No.
- ACC-003
- 規格 Size
- 50 μl
Description
Anti-Cav1.2
| Product#: | ACC-003 |
| Sizes: |
| 50 ml |
| 0.2 ml |
All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptor. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channels blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#C-650), Calciseptine (#C-500) and FS-2 (#F-700).
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)TTKI NMDDL QPSEN EDKS (CNC1), corresponding to residues 848-865 of rat Cav1.2 (Accession P22002). |
| Putative epitope location: |
Intracellular loop between II and III domains. |
 |
| Homology: |
Mouse - identical; guinea pig (17/18 residues identical); human, rabbit (16/18 residues identical). |
| Reactivity Confirmed: |
Rat, mouse, rabbit,3 human.4,5 |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-Cav1.2 antibody (1:200). 2. Anti-Cav1.2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of Cav1.2 in Mouse Brain. |
 |
| Immunohistochemical staining of Cav1.2 channel with Anti-Cav1.2 antibody (#ACC-003) in mouse cerebellum. (A) Cav1.2 channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows). (B) staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) in the section demonstrates the location of dendrites in the molecular layer. (C) Confocal merge of Cav1.2 and CBD28K. |
| References: |
| 1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579. |
| 2. http://www.iuphar-db.org/iuphar-ic/calcium.html |
| 3. Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337. |
| 4. Kreuzberg, U. et al. (2000) Am J. Physiol. 278, H723. |
| 5. Allard, B. et al. (2000) J. Biol. Chem. 275. 25556. |