Anti-Cav1.2-ATTO-488
- 貨號 Cat. No.
- ACC-003-AG
- 規格 Size
- 50 μl
Description
Anti-Cav1.2-ATTO-488
| Product#: | ACC-003-AG |
| Sizes: |
| 50 µl |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)TTKI NMDDL QPSEN EDKS (CNC1), corresponding to residues 848-865 of rat Cav1.2 (Accession P22002). |
| Putative epitope location: |
Intracellular loop between II and III domains. |
| Homology: |
Mouse - identical; guinea pig (17/18 residues identical); human, rabbit (16/18 residues identical). Label: ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range.. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain membranes using unlabeled Anti-Cav1.2 antibody (#ACC-003): 1. Anti-Cav1.2 antibody (1:200). 2. Anti-Cav1.2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Cav1.2 in rat cerebellum |
 |
Immunohistochemical staining of paraffin embedded section of rat pancreas using Anti-Cav1.2-ATTO-488 antibody (#ACC-003-AG) (1:50). Staining is highly specific for endocrine cells of the Isle of Langerhans (IL). Expression of Cav1.2 in rat pancreas |
 |
Immunohistochemical staining of rat cerebellum frozen section using Anti-Cav1.2-ATTO-488 antibody (#ACC-003-AG) (1:100). Both dendrites of Purkinje cells (horizontal arrows) and fibers of Bergmann glia (vertical arrows) were stained. DAPI (blue Nissl counterstain) helps define the layers: granule (G), Purkinje (P) and molecular (M) |
| Immunocytochemistry: |
Expression of Cav1.2 in mouse pancreatic microvascular endothelial cells (MS1) |
 |
Immunocytochemical staining of MS1 cells using Anti-Cav1.2-ATTO-488 antibody (#ACC-003-AG) (1:50). A, Intracellular staining of Paraformaldehyde-fixed and permeabilized MS1 cells. B, The cell-permeable dye Hoechst 33342 (blue) was used for nuclear staining. C, Merged images of panels A and B. |
| References: |
| 1. Catterall, W.A. et al. (2003) Pharmacol. Rev.55, 579. |
| 2. http://www.iuphar-db.org/iuphar-ic/calcium.html. |
| 3. Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337. |
| 4. Kreuzberg, U. et al. (2000) Am J. Physiol. 278, H723. |
| 5. Allard, B. et al. (2000) J. Biol. Chem. 275. 25556. |
All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptor. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channels blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#C-650), Calciseptine (#C-500) and FS-2 (#F-700).
Alomone Labs is pleased to offer a new version of this antibody, Anti-Cav1.2-ATTO-488 antibody (#ACC-003-AG), that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. The Anti-Cav1.2-ATTO-488 antibody has been tested in immunohistochemical and immunocytochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.