Anti-CaMPARI-Red [4F6]

CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time. CaMPARI 2.0 is the second generation of CaMPARI molecules with improve green brightness by 50% and red brightness by 250%. Additionally, CaMPARI 2.0 has faster kinetics, and a lower photoconversion rate in low calcium conditions resulting in a net 100-fold difference in green-to-red switching in low- vs. high-calcium conditions. Lastly, these different CaMPARI proteins display a range of sensitivity in their calcium binding (Kd’s ranging from 100 nM to 1 uM of free calcium). The variable domain region of the original mouse IgG1 antibody was reformatted to generate the Fab fragment and the chimeric rabbit IgG.