Anti-CAMK2 Antibody

Anti-CAMK2 Antibody LS-B3743

CAMK2 Rabbit anti-Rat Polyclonal () Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3743 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3743 was determined to be 10 ug/ml. Learn more

CAMK2 Antibody Details

 

 

Target:

Rat CAMK2

 

Host:

Rabbit

 

Reactivity:

Bovine, Guinea pig, Hamster, Human, Mouse, Rat

 

Clonality:

 Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Western blot (WB) (1:1000)

 

Optimal dilution to be determined by the researcher.

 

CAMK2 Antibody Specificity

 

Immunogen:

CAMK2 antibody was raised against a 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (residues 7-20) and the peptide coupled to KLH.

 

Specificity:

Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.

CAMK2 Antibody Packaging

 

Presentation:

BBS, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

 

Storage:

Store at -20°C.

 

Restrictions:

For research use only.