Anti-Calcium Sensing Receptor (extracellular)
- 貨號 Cat. No.
- ACR-004
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Calcium Sensing Receptor (extracellular)
| Product#: | ACR-004 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR (Accession P41180). |
 |
| Putative epitope location: |
N-terminus, Extracellular. |
| Homology: |
Rat, mouse, bovine, and canis ?identical. |
| Reactivity Confirmed: |
| Human, rat, and mouse. |
Applications:
| Western Blotting: |
 | Western blotting of human Colo205 (lanes 1 and 3), rat liver (lanes 2 and 4), and mouse kidney (lanes 5 and 6) lysates: 1, 2, 5. Anti-CaSR (extracellular) antibody (#ACR-004), (1:200). 3, 4, 6. Anti-CaSR (extracellular) antibody preincubated with the control peptide antigen. |
| Immunocytochemistry: |
| Expression of Calcium sensing receptor (CaSR) in C6 (rat brain glioma) cells. |
 |
Immunocytochemical staining of CaSR in live intact C6 cells. Paraformaldehyde-fixed and permeabilized C6 cells were stained as described. A. Immunocytochemical staining using Anti-CaSR (extracellular) antibody (#ACR-004), (1:50) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. |
| Flow Cytometry: |
Flow cytometry analysis of live Jurkat (human acute T cell leukemia) cells |
 |
____ Unstained Jurkat cells ____ Anti-CaSR (extracellular) antibody (#ACR-004) (10mg per 5x105 cells). |
| References: |
| 1. IUPHAR RECEPTOR DATABASE. |
| 2. Racz, G.Z. et al. (2002) Gut 51, 705. |
| 3. Canaff, L. and Hendy, G.N. (2002) J. Biol. Chem. 277, 30337. |
| 4. Chen, R.A. and Goodman, W.G. (2004) Am. J. Physiol. Renal Physiol. 286, F1005. |
| 5. Chattopadhyay, N. (2006) Am. J. Physiol. Endocrinol. Metab. 290, E761. |
The Calcium-Sensing Receptor (CaSR) is a member of subfamily C of the G-protein-coupled receptor (GPCR) superfamily. As its primary role, the CaSR is a key player in calcium homeostasis and is expressed in tissues involved in calcium metabolism, such as parathyroid cells and kidney.1,2
The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and pancreas. However, the role of CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR.3
The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and pancreas. However, the role of CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR.3
CaSR activation is followed by a rapid, transient increase in the cytosolic calcium concentration, resulting from mobilization of calcium from thapsigargin-sensitive intracellular stores, as well as an increased calcium influx through voltage-insensitive calcium channels located at the cell membrane.4
CaSR regulates cellular processes such as proliferation, apoptosis, and differentiation under both normal and pathologic conditions.5
Recent studies demonstrated the expression in human colon epithelium of CaSR, which regulates proliferation and differentiation. In colon carcinomas,lower levels of expression were found in compared to normal colon tissue.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellularepitope at the N-terminus of human CaSR. The Anti-CaSR (extracellular) antibody (#ACR-004) can be used in Western blotting analyses and recognizes human, rat, and mouse CaSR samples.