Anti-BRCC3 / BRCC36 Antibody

Anti-BRCC3 / BRCC36 Antibody LS-B3588

BRCC3 / BRCC36 Mouse anti-Human Monoclonal (AT3B1) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3588 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3588 was determined to be 5 ug/ml. Western Blot: Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Learn more

BRCC3 / BRCC36 Antibody Details

Target: Human BRCC3 / BRCC36

Host: Mouse

Reactivity: Human

Clonality: IgG1,k Monoclonal [AT3B1] 

Purification: Protein G Column

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1:1000 - 1:2000)

ELISA

Optimal dilution to be determined by the researcher.

 

BRCC3 / BRCC36 Antibody Specificity

Immunogen: BRCC3 / BRCC36 antibody was raised against recombinant BRCC36 (1-316aa) purified from E. coli

Specificity: BRCC36 (1-316aa)

BRCC3 / BRCC36 Antibody Packaging

Presentation: PBS, pH 7.4, 0.1% sodium azide

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For research use only.