Anti-Bone Alkaline Phosphatase Antibody

Anti-Bone Alkaline Phosphatase Antibody LS-B3666

Bone Alkaline Phosphatase Mouse anti-Human Monoclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3666 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3666 was determined to be 10 ug/ml. Suitable for use in ELISA and Western Blot. Western Blot: Stains a band of bone AP at ~90kD. Learn more

Bone Alkaline Phosphatase Antibody Details

 

 

Target:

Human Bone Alkaline Phosphatase

 

Host:

Mouse

 

Reactivity:

Bovine, Human

 

Clonality:

IgG1 Monoclonal

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Western blot (WB)

 

ELISA

 

Optimal dilution to be determined by the researcher.

 

Bone Alkaline Phosphatase Antibody Specificity

 

Immunogen:

Bone Alkaline Phosphatase antibody was raised against purified alkaline phosphatase (native) from human bone.

 

Specificity:

Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.

Bone Alkaline Phosphatase Antibody Packaging

 

Presentation:

PBS, pH 7.2, 0.09% sodium azide

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.