Anti-Bombesin Receptor 2 (extracellular)
- 貨號 Cat. No.
- ABR-002
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Bombesin Receptor 2 (extracellular)
| Product#: | ABR-002 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide(C)RSYHYSEVDTSMLH corresponding to residues 287-300 of human BB2 (Accession P30550). |
 |
| Putative epitope location: |
3rd extracellular loop. |
| Homology: |
| Rat, mouse, dog - identical. |
| Reactivity Confirmed: |
| Human,rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of human DU-145 (lanes 1 and 3) and PC-3 (lanes 2 and 4) prostate carcinoma cell lines: 1, 2. Anti-BB2 antibody (#ABR-002) (1:200). 3, 4. Anti-BB2 antibody, preincubated with the control peptide antigen. |
 | Western blotting of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1, 2. Anti-BB2 antibody (#ABR-002) (1:500). 3, 4. Anti-BB2, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
Immunohistochemical staining of Bombesin Receptor 2 in human colon cancer sections. Longitudinal sections of paraffin-embedded human colon showing malignant growth (A and B) and normal colon (C and D). Staining is specific for absorptive epithelial cells in the crypts of Lieberkuhn (C and D) and for epithelium- derived malignant cells (A and B). After citrate treatment in a pressure cooker for antigen retrieval, slides were incubated overnight at 4èš“ with Anti-Bombesin Receptor 2 antibody (#ABR-002) (1:50). Histofine (pink) was used for the color reaction. Counterstain is Hematoxilin. |
| Immunocytochemistry: |
 |
| Immunocytochemical staining of live intact human HT-29 (colorectal adenocarcinoma) cells with Anti-BB2 antibody (#ABR-002)(1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody showing surface expression of the BB2 receptor. |
| References: |
| 1. Battey, J.F. et al. (1991) Proc Natl Acad Sci USA 88, 395. |
| 2. Kroog, G.S. et al.(1995) Med. Res. Rev. 15, 389. |
| 3. Ohki-Hamazaki, H. et al. (2005) Int. J. Dev. Biol. 49, 293. |
| 4. Reubi, J.C. (2003) Endocr. Rev. 24, 389. |
Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP; a 27 amino acid homologue) and neuromedin B (NMB; a 10 amino acid homologue).
BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R.1 BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified.
All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.2
GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestinal tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes.2 In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation.3
Finally, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4