Anti-BNIP3 Antibody

Anti-BNIP3 Antibody LS-B3305

BNIP3 Mouse anti-Human Monoclonal (aa112-124) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3305 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3305 was determined to be 10 ug/ml. Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 2-4 ug/ml. Detects a band at ~30kD and ~60kD. Positive control: Whole cell lysate from 293T cells transfected with BNIP3. Learn more

BNIP3 Antibody Details

 

 

Target:

Human BNIP3

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG2b Monoclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Western blot (WB)

 

Immunoprecipitation (IP)

 

ELISA

 

Optimal dilution to be determined by the researcher.

 

BNIP3 Antibody Specificity

 

Immunogen:

BNIP3 antibody was raised against recombinant fragment (Residues 1-163 of Human BNIP3). Epitope: The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

 

Epitope:

aa112-124

 

Specificity:

Species cross-reactivity: Cross-reacts with Human.

BNIP3 Antibody Packaging

 

Presentation:

PBS, pH 7.4, 15mM sodium azide

 

Storage:

4°C while lyophilized, once reconstituted, aliquot and store at -70°C.

 

Restrictions:

For research use only.