Anti-BMI-1 Antibody

Anti-BMI-1 Antibody LS-B346

BMI-1 Goat anti-Human Polyclonal (aa252-264) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B346 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D3, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B346 was determined to be 2.5 ug/ml. Learn more

BMI-1 Antibody Details

 

 

Target:

Human BMI-1

 

Host:

Goat

 

Reactivity:

Bovine, Cat, Chimpanzee, Chicken, Dog, Frog, Human, Mouse, Orangutan, Rat

 

Clonality:

 Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (2.5 µg/ml)

 

Western blot (WB) (1:500 - 1:3000)

 

ELISA (1:5000 - 1:30000)

 

Optimal dilution to be determined by the researcher.

 

BMI-1 Antibody Specificity

 

Immunogen:

BMI1 antibody was raised against synthetic peptide from human BMI1.

 

Epitope:

aa252-264

 

Specificity:

Amino acids 252-264 of human Bmi1 protein.

BMI-1 Antibody Packaging

 

Presentation:

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.

 

Restrictions:

For research use only.