Anti-BCL2 / Bcl-2 Antibody

Anti-BCL2 / Bcl-2 Antibody LS-B3319

BCL2 / Bcl-2 Mouse anti-Human Monoclonal (aa41-54) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3319 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3319 was determined to be 1:50. Western Blot: 1-2 ug/ml for two hours at RT. Immunoprecipitation: 2 ug antibody/mg protein lysate using Protein G. Positive control: Raji Cells. Tonsil and follicular lymphomas. Learn more

BCL2 / Bcl-2 Antibody Details

 

 

Target:

Human BCL2 / Bcl-2

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG1,k Monoclonal

 

Purification:

Protein G Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (1:50)

 

Western blot (WB) (1 µg/ml)

 

Immunoprecipitation (IP)

 

Flow Cytometry (Flo)

 

Immunofluorescence (IF)

 

ELISA

 

Optimal dilution to be determined by the researcher.

 

BCL2 / Bcl-2 Antibody Specificity

 

Immunogen:

BCL2 antibody was raised against synthetic peptide corresponding to residues 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

 

Epitope:

aa41-54

 

Specificity:

Recognizes human Bcl-2a at ~25kD. Species Reactivity: Human. Does not react with mouse or rat.

BCL2 / Bcl-2 Antibody Packaging

 

Presentation:

PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.