Anti-BAD Antibody

Anti-BAD Antibody LS-B3293

BAD Mouse anti-Mouse Monoclonal (N-Terminus) (BYC001) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3293 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3293 was determined to be 10 ug/ml. Western Blot: Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system. The endogenous level of Bad in most lysates is below the detectable limits of this antibody. Immunoprecipitation: 4 ug immunoprecipitates Bad which was subsequently detected using anti-phospho Bad. Learn more

BAD Antibody Details

 

 

Target:

Mouse BAD

 

Host:

Mouse

 

Reactivity:

Human, Mouse

 

Clonality:

IgG Monoclonal [BYC001]

 

Purification:

Protein G Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Western blot (WB)

 

Immunoprecipitation (IP)

 

Optimal dilution to be determined by the researcher.

 

BAD Antibody Specificity

 

Immunogen:

BAD antibody was raised against full-length murine Bad containing a N-terminal His6-tag.

 

Epitope:

N-Terminus

 

Specificity:

Recognizes human Bad, Mr 23kD, and mouse Bad, Mr 31kD. Occasionally a Mr 14kD protein is detected in human lysates.

BAD Antibody Packaging

 

Presentation:

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.