Anti-B1 Bradykinin Receptor
- 貨號 Cat. No.
- ABR-011
- 規格 Size
- 50 μl 0.2ml
Description
Anti-B1 Bradykinin Receptor
| Product#: | ABR-011 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
| Peptide (C)KEAS RTR*SG GPKGS K corresponding to amino acid residues 243-257 of rat BKRB1 with replacement of cysteine 250 (C250) with serine (*S) (Accession P97583). |
 |
| Putative epitope location: |
| 3rd intracellular loop. |
| Homology: |
Mouse ?13/15 amino acid residues identical. Human - unlikely to recognize human samples. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of B1 Bradykinin receptor in rat brain (lanes 1 and 3) and rat heart (lanes 2 and 4) lysates: 1. Anti-B1 Bradykinin receptor antibody (#ABR-011), (1:200). 2. Anti-B1 Bradykinin receptor (BKRB1) antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of B1 Bradikinin Receptor in rat CNS |
 |
Immunohistochemical staining of rat brain frozen section (A&B) and cerebellum (C) using Anti-B1 Bradykinin receptor antibody (#ABR-011), (1:50). B1 Bradykinin receptor is expressed in neuronal cells of the amygdala (C) and hippocampus (CA1) (B) as well as in the axons of Purkinje cells in the cerebellum (arrowheads on C). Counterstain is Hoechst 33342. |
Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function.1
Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). The desArg9-BK and Lys-desArg9-BK peptides activate BKRB1 while BK and Lys-BK operate by activating BKRB2.2 Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.
Expression of BKRB1is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. BKRB1 was long considered not to be expressed in healthy tissues. However, recent work has demonstrated a low level of expression of BKRB1 in the CNS of rodent and primates. In contrast, BKRB2 is constitutively expressed on various cell types.3 BKRB1 represents a potential therapeutic target for treatment of inflammatory disorders and cardiovascular diseases.
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Bradykinin 1 receptor (BKRB1). The Anti-B1 Bradykinin receptor antibody (#ABR-011) can be used in Western blot analyses and Immunohistochemical applications. It has been designed to recognize B1 Bradykinin receptor from rat and mouse samples.