Anti-B-Raf Antibody

Anti-B-Raf Antibody LS-B3444

B-Raf Mouse anti-Human Monoclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3444 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3444 was determined to be 1:200. Learn more

B-Raf Antibody Details

 

 

Target:

Human B-Raf

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

 Monoclonal

 

Purification:

Ascites

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (1:200)

 

Western blot (WB) (1: 500 - 1: 1000)

 

ELISA (1: 10000)

 

Optimal dilution to be determined by the researcher.

 

B-Raf Antibody Specificity

 

Immunogen:

BRAF antibody was raised against recombinant human BRAF.

 

Specificity:

Ni-NTA purified truncated recombinant BRAF expressed in E. coli strain BL21 (DE3).

B-Raf Antibody Packaging

 

Storage:

Long term: -20°C; Short term: -20°C

 

Restrictions:

For research use only.