Anti-B-Raf Antibody

Anti-B-Raf Antibody LS-B3862

B-Raf Mouse anti-Human Monoclonal (1H12) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3861 was determined to be 1:200. Learn more

B-Raf Antibody Details

 

 

Target:

Human B-Raf

 

Host:

Mouse

 

Reactivity:

Human, Mouse

 

Clonality:

IgG1 Monoclonal [1H12]

 

Purification:

Ascites

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (1:200 - 1:300)

 

Western blot (WB) (1:500 - 1:2000)

 

Immunofluorescence (IF) (1:200 - 1:1000)

 

ELISA (1:10000)

 

Optimal dilution to be determined by the researcher.

 

B-Raf Antibody Specificity

 

Immunogen:

B-Raf antibody was raised against purified recombinant fragment of human BRAF (aa 285-401) expressed in E. coli

 

Specificity:

Human BRAF

B-Raf Antibody Packaging

 

Presentation:

Ascites fluid, 0.03% sodium azide

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.