Anti-ASIC4
- 貨號 Cat. No.
- ASC-015
- 規格 Size
- 50 μl 0.2ml
Description
Anti-ASIC4
| Product#: | ASC-015 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide CKIKFAEEDAKPKEKEAGDE corresponding to residues 7-26 of rat ASIC4 (Accession Q9QYV9). |
 |
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
Mouse, human -identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blotting of ND7/23 cell line (mouse neuroblastoma x rat DRG neuron hybrid cell line) (1 and 3) and rat brain (2 and 4) lysate: 1, 2. Anti-ASIC4 antibody (1:200). 3, 4. Anti-ASIC4 antibody, preincubated with the control peptide antigen. |
 | Western blotting of rat dorsal root ganglion (DRG) lysate: 1. Anti-ASIC4 antibody (1:200). 2. Anti-ASIC4 antibody, preincubated with the control peptide antigen. |
Control antigen included in price
| References: |
1. | Grunder, S. et al. (2000) Neuroreport 11, 1607. |
2. | Akopian, A.N. et al. (2000) Neuroreport 11, 2217. |
3. | Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735 |
Acid-sensing ion channels (ASICs) are Na+ channels activated by external protons. ASIC4 is a member of the ASIC family that includes another three members: ASIC1, ASIC2 and ASIC3. The ASIC family is in fact part of a large channel superfamily known as degenerin/epithelial Na+ channels (DEG/ENaC) and share the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini.
The functional ASIC channel is composed of 4 subunits, which can form either a homo or heterotetramer. The subunit composition of the tetrameric channel will determine its biophysical properties such as pH sensitivity, inactivation kinetics and channel pharmacology. In fact, the ASIC4 protein does not appear to function as a proton-gated channel when expressed alone in heterologous systems and therefore a modulatory role for this subunit has been proposed.
Expression of the ASIC4 protein has been detected in brain, pituitary gland, spinal cord and inner ear. The functional significance of ASIC4 expression in these tissues still awaits clarification.
We are now offering a new ASIC4 antibody directed against a highly specific region in the intracellular N-terminus domain of the rat ASIC4 protein. The antibody will also work well with mouse and human samples.