Anti-ARID5A Antibody

 

Anti-ARID5A Antibody LS-B3563

ARID5A Rabbit anti-Human Polyclonal (C-Terminus) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3563 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3563 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Learn more

ARID5A Antibody Details

 

 

Target:

Human ARID5A

 

Host:

Rabbit

 

Reactivity:

Human, Mouse, Rat

 

Clonality:

IgG Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml)

 

Western blot (WB)

 

Optimal dilution to be determined by the researcher.

 

ARID5A Antibody Specificity

 

Immunogen:

ARID5A antibody was raised against synthetic peptide from C-terminus of human ARID5A (NP_997646.1, Q03989). Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%); Pig (93%); Horse (91%); Guinea pig (85%).

 

Epitope:

C-Terminus

 

Specificity:

Human ARID5A

ARID5A Antibody Packaging

 

Presentation:

Lyophilized from PBS with 2% sucrose

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.