Anti-Aquaporin 9
- 貨號 Cat. No.
- AQP-009
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Aquaporin 9
| Product#: | AQP-009 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide(C)EKDGAKKSLMQRLALKcorresponding to residues 4-19 of rat AQP-9 (Accession number P56627). |
 |
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
Mouse 13/16 residues identical. Unlikely to recognize human samples. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain (lanes 1and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes: 1,2,3. Anti-Aquaporin 9 antibody (#AQP-009), 1:200. 4,5,6. Anti-Aquaporin 9, preincubated with the control peptide antigen. |
 | Western blot analysis of rat H4-II-E-C3 hepatoma cell line lysate: 1. Anti- Aquaporin 9 antibody (#AQP-009), 1:200. 2. Anti- Aquaporin 9, preincubated with the control peptide antigen. |
 | Western blot analysis of mouse brain membranes: 1. Anti- Aquaporin 9 antibody (#AQP-009), 1:200. 2. Anti- Aquaporin 9, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of Aquaporin 9 in rat liver |
 |
Immunohistochemical staining of paraffin embedded sections of rat liver using Anti-Aquaporin 9 antibody (#AQP-009) (1:100). Aquaporin 9 (brown staining) is expressed in hepatocytes of the liver parenchyma. Counterstain is Hematoxilin. Expression of Aquaporin 9 in rat epididymus |
 |
Immunohistochemical staining of paraffin embedded section of rat tests using Anti-Aquaporin 9 antibody (#AQP-009) (1:100). Aquaporin 9 (brown staining) is expressed in the columnar epithelium of the epididymus (arrows). Counterstain is Hematoxilin. |
Aquaporin 9 (AQP-9) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include AQP-1, AQP-2, AQP4, AQP-5, AQP-6 and AQP-8, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.2
AQP-9 is expressed in several tissues including brain, liver, testis and spleen.
In the liver AQP-9 has a central role in glycerol metabolism as demonstrated in experiments using AQP-9 knockout mice: mice deficient in AQP-9 expression showed enhanced plasma levels of glycerol and triglycerides.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular N-terminus domain of the rat AQP-9 channel. Anti-Aquaporin 9 antibody (#AQP-009) can be used in Western blot and immunohistochemical applications, and recognizes AQP-9 from rat and mouse samples.