Anti-Aquaporin 8
- 貨號 Cat. No.
- AQP-008
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Aquaporin 8
| Product#: | AQP-008 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide(C)REIKGKETNMADSYHcorresponding to residues 14-28 of rat AQP-8 (Accession number P56405). |
 |
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
| Unlikely to recognize mouse or human samples. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat small intestine membranes: 1. Anti-AQP-8 antibody (#AQP-008), 1:200. 2. Anti-AQP-8 preincubated with the control peptide antigen. |
 | Western blot analysis of rat pancreas membranes:
|
| Immunohistochemistry: |
 |
Expression of Aquaporin 8 in rat pancreas. Immunohistochemical staining was performed in paraffin-embedded sections of rat pancreas. Staining (brown color) is specific for the apical portion of acinar cells in the exocrine pancreas. After Tris-EDTA treatment for antigen retrieval, slides were incubated overnight at 4èš“ using Anti-Aquaporin 8 antibody(#AQP-008), (1:100). Hematoxylin is used as the counterstain. |
 |
Expression of Aquaporin 8 in rat lung. Immunohistochemical staining was performed in paraffin-embedded sections of rat lung. Staining (brown color) is specific for the bronchiolar epithelium. After Tris-EDTA treatment for antigen retrieval, slides were incubated overnight at 4èš“ using Anti-Aquaporin 8 antibody (#AQP-008), (1:100). Hematoxylin is used as the counterstain. |
| References: |
| 1. Koyama, Y.et al.(1997) J Biol Chem 272, 30329. |
| 2. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. |
| 3. Ma, T. and Verkman, A.S. (1999) J. Physiol. 517, 317. |
| 4. Laforenza, U. et al. (2005) J. Nutr. 135, 2329. |
| 5. Zahn, A. et al. (2007) World J. Gastroenterol. 13, 1687. |
Aquaporin 8 (AQP-8) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include besides AQP-8, AQP-1, AQP-2, AQP4 and AQP-5, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.2
AQP-8 is expressed along the gastrointestinal tract including small intestine, colon, pancreas and liver and also in other organs such as testis, lung and kidney.
The physiological function of AQP-8 is not entirely clear but studies suggest that it has an important role in colonic water adsorption and hepatocyte bile formation.3,4 Interestingly, AQP-8 expression is markedly altered in gastrointestinal disorders such as ulcerative colitis and inflammatory bowel disease.5