Anti-Aquaporin 7
- 貨號 Cat. No.
- AQP-007
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Aquaporin 7
| Product#: | AQP-007 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptidecorresponding to residues 7-24 of rat AQP-7 (Accession number P56403). |
 |
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
Mouse 15/18 residues identical; Human 11/18 residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of mouse (lanes 1 and 3) and rat (lanes 2 and 4) kidney membranes: 1, 2. Anti-Aquaporin 7 antibody, 1:500. 3, 4. Anti-Aquaporin 7 antibody, preincubated with the control peptide antigen. |
| Immunocytochemistry: |
 |
Immunocytochemical staining of 3T3-L1 adipocytes with Anti-Aquaporin 7 antibody (1:800), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red) (A). (C) Anti-Aquaporin 7 antibody, preincubated with the control peptide antigen followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody. (B) and (D) show visible light images of the adipocytes shown on (A) and (C) respectively. |
| References: |
| 1. Kuriyama, H. et al. (1997) Biochem. Biophys. Res. Commun. 241, 53. |
| 2. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. |
| 3. Fruhbeck, G. et al. (2006) Trends Pharmacol. Sci. 27, 345. |
Aquaporin 7 (AQP-7) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.
The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-7, AQP-3, AQP-9 and AQP-10.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.
AQP-7 is expressed in ovary, testis, kidney and adipose tissue.
The function of AQP-7 in adipose tissue attracted much interest as mice deficient in AQP-7 developed adult-onset obesity and type 2 diabetes. AQP-7 modulates adipocyte glycerol permeability thereby controlling triglyceride accumulation and fat cell size.