Anti-Aquaporin 4
- 貨號 Cat. No.
- AQP-004
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Aquaporin 4
| Product#: | AQP-004 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
GST fusion protein with the sequence EYVFC PDVEL KRRLK EAFSK AAQQT KGSYM EVEDN RSQVE TEDLI LKPGV VHVID IDRGD EKKGK DSSGE VLSSV, corresponding to residues 249-323 of rat AQP4 (Accession # P47863) |
 |
| Putative epitope location: |
| Intracellular, C-terminus. |
| Homology: |
mouse, bovine, human, and rabbit - 73/75, 71/75, 69/75, and 64/75 residues identical respectively. |
| Reactivity Confirmed: |
Rat, human. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-Aquaporin 4 antibody (#AQP-004) (1:1000). 2. Anti-Aquaporin 4, preincubated with the control fusion protein. |
| References: |
| 1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. |
| 2. Manley, G.T. et al. (2004) Neuroscience. 129, 983. |
| 3. Lennon, V.A. et al. (2005) J. Exp. Med. 202, 473. |
Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belongs to the first group.1
Little is known about the function of the two newest members, AQP-11 and AQP-12.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1
AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance.2
In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-4.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of rat Aquaporin 4 channel. Anti- Aquaporin 4 antibody (#AQP-004) can be used in Western blot and immunohistochemical applications, and recognizes the Aquaporin 4 channel from rat, mouse and human samples.