Anti-Aquaporin 2
- 貨號 Cat. No.
- AQP-002
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Aquaporin 2
| Product#: | AQP-002 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)RQSVELHSPQSLPRGSKA, corresponding to residues 254-271 of rat AQP2 (Accession number P34080). |
 |
| Putative epitope location: |
Intracellular, C-terminus. |
| Homology: |
| Mouse -identical; human, sheep - 17/18 residues identical. |
| Reactivity Confirmed: |
Rat, human. |
Applications:
| Western Blotting: |
 | Western blotting of rat kidney membranes 1. Anti-Aquaporin 2 antibody (#AQP-002) (1:200). 2. Anti-Aquaporin 2, preincubated with a control peptide antigen. |
| Immunohistochemistry: |
 |
Staining of Aquaporin 2 in rat kidney. Paraffin embedded section of rat kidney showing the inner medulla. Slides were stained with Anti-Aquaporin 2 antibody (#AQP-002) (1:100). Intense stain (brown color) is present in collecting ducts but not in thin segments of the loop of Henle. Counterstain is Hematoxylin. |
| References: |
| 1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. |
| 2. Deen, P.M. et al. (1994) Science 264, 92. |
| 3. Nielsen, S. et al. (2002) Physiol. Rev. 82, 205. |
| 4. Robben, J.H. et al. (2006) Am. J. Physiol. Renal Physiol. 291, F257. |
Aquaporin 2 (AQP-2) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-2 together with AQP-1, AQP-4 and AQP-5 belongs to the first group.1
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1
AQP-2 expression is largely confined to the kidney, particularly in the renal collecting duct where it performs a key role in water absorption and urine concentration. In fact, mutations in the AQP-2 gene produce hereditary nephrogenic diabetes insipidus, a disorder that results in the excretion of large volumes of urine.2
Under normal conditions, water homeostasis in the kidney is regulated through the anti-diuretic hormone vasopressin. Vasopressin is secreted from the pituitary gland and transported to the kidney through the blood where it binds to its receptor that is mainly expressed in cells of the collecting duct. The activated vasopressing receptor induces an increase in intracellular cAMP and subsequent PKA activation, which phosphorylates AQP-2. This phosphorylation causes the translocation of AQP-2 channels from intracellular vesicles to the cell membrane where it markedly increases water permeability.1, 3, 4
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of the rat Aquaporin 2 channel. Anti- Aquaporin 2 antibody (#AQP-002) can be used in Western blot and immunohistochemical applications, and recognizes the Aquaporin 2 channel from rat, mouse and human samples.