Anti-Aquaporin 1
- 貨號 Cat. No.
- AQP-001
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Aquaporin 1
| Product#: | AQP-001 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide(C)KVWTSGQVEEYDLDADDIN corresponding to residues 242-260 of human AQP1 (accession number P29972). |
 |
| Putative epitope location: |
| Intracellular, C-terminus. |
| Homology: |
rat, mouse, cattle, sheep - identical; dog - 18/19 residues identical; frog (Rana esculenta), toad (Bufo marinus) - 14/19 residues identical. |
| Reactivity Confirmed: |
| rat, human |
Applications:
| Western Blotting: |
 | Western blotting of rat kidney membranes: 1. Anti-Aquaporin 1 antibody (AQP-001), 1:400 2. Anti-Aquaporin 1, preincubated with the control peptide antigen. |
| References: |
| 1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. |
| 2. Ma, T. et al. (1998) J Biol Chem 273, 4296. |
| 3. Hu. J. and Verkman, A.S. (2006) FASEB J. 20, 1892. |
Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2, AQP-4 and AQP-5 belongs to the first group.1
Little is known about the function of the two newest members, AQP-11 and AQP-12.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1
AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration.2 In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential. 3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of the human Aquaporin 1 channel. Anti- Aquaporin 1 antibody (#AQP-001) can be used in Western blot and immunohistochemical applications, and recognizes the Aquaporin 1 channel from rat, mouse and human samples.