Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488
- 貨號 Cat. No.
- AAR-012-AG
- 規格 Size
- 50 μl
Description
Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO-488
| Product#: | AAR-012-AG |
| Sizes: |
| 50 µl |
| Host: |
| Rabbit. |
| Epitope: |
PeptideDNLNATGTNESAFNC corresponding to amino acid residues 21-35 of rat AT2 Receptor (Accession P35351). |
 |
| Putative epitope location: |
| Extracellular, N-terminal domain. |
| Homology: |
Mouse - identical. Specificity: Wonî–¹ recognize human AT2 Receptor. Label: Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC . |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Immunohistochemistry: |
Immunohistochemical staining of AT2 receptorin rat brain. |
 |
Rat frozen free floating sections were stained with Anti-AT2 Receptor (extracellular)-ATTO-488 (#AAR-012-AG) antibody (1:20). The AT2 receptor (green) was detected in neurons in the vicinity of the hypothalamic paraventricular nucleus (PVN). In some neurons (thick white arrow), axonal processes with varicosities were observed (thin white arrow). Nuclei were visualized with DAPI counterstain (blue). |
| Immunocytochemistry: |
Immunocytochemical staining of intact live mouse 3T3-L1 cells with Anti-AT2 Receptor(extracellular)-ATTO-488 (#AAR-012-AG). |
 |
Staining of intact live mouse 3T3-L1 cells with Anti-AT2 Receptor (extracellular)-ATTO-488 (#AAR-012-AG) antibody (green)(1:50). Live view of the same field was superimposed to the fluorescent one. |
Angiotensin II receptor type 2 or AT2 receptor is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II).
Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.
Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension.
Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Gia2 and the Gia3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1.
AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the Angiotensin II Receptor Type-2. The antibody can be used in immunocytochemistry, immunohistochemistry and Western blot analysis and will recognize AT2 receptor from rat and mouse samples.
We are pleased to offer a new version of this antibody that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
The antibody has been tested in immunocytochemical and immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.