Anti-Angiotensin II Receptor Type-1 (extracellular)
- 貨號 Cat. No.
- AAR-011
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Angiotensin II Receptor Type-1 (extracellular)
| Product#: | AAR-011 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
PeptideNSSTEDGIKRIQDDC corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). |
 |
| Putative epitope location: |
Extracellular, N-terminal domain. |
| Homology: |
Mouse, rat, rabbit, dog- identical. |
| Reactivity Confirmed: |
Human, rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes: 1, 2. Anti-AT1 Receptor(extracellular) antibody (#AAR-011) (1:200). 3, 4. Anti-AT1 Receptor (extracellular), preincubated with the control peptide antigen. |
 | Western blot analysis of mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes: 1, 2. Anti-AT1 Receptor(extracellular) antibody, (1:500). 3, 4. Anti-AT1 Receptor (extracellular), preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
| Immunohistochemical staining of Angiotensin II Receptor type-1 (AT1receptor) in mouse cerebellum with Anti-AT1 Receptor (extracellular) antibody (#AAR-011). AT1 receptor was present in the Purkinje layer (green). White arrows point at AT1 receptor immunoreactive cells. Staining of the same section with mouse anti-Parvalbumin (red) revealed partial co-localization (C). |
| Immunocytochemistry: |
| Immunocytochemical Staining of live intact rat C6 glioma cells. |
 |
A. Immunocytochemical Staining of live intact rat C6 glioma cells using Anti-AT1 receptor(extracellular) antibody (#AAR-011) 1:100, followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody. B. Live intact C6 cells. |
| Flow Cytometry: |
FACS analysis of live intact human Jurkat T-cell leukemia cells: |
 |
_____ Jurkat cells + Goat-anti-Rabbit-FITC alone. _____Jurkat cells + Anti-AT1 receptor (extracellular) (5 ç—¢) + Goat-anti-Rabbit-FITC. _____ Jurkat cells + Anti-AT1 receptor (extracellular) (10 ç—¢) + Goat-anti-Rabbit-FITC. |
| References: |
| 1. Sasaki, K. et al. (1991) Nature 351, 230. |
| 2. Murphy, T.J. et al. (1991) Nature 351, 233. |
| 3. de Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415. |
| 4. Hunyady, L. and Catt, K.J. (2006) Mol. Endocrinol. 20, 953. |
Angiotensin II receptor type 1 or AT1 receptor is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II).
Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.
Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration.
In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc.
As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the Angiotensin II Receptor Type-1. This antibody can be used in Western blot, Immunohistochemistry, Immunocytochemistry and Indirect Flow Cytometry applications and will recognize Angiotensin II Receptor Type-1 from rat, mouse and human samples.